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Image Search Results
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression
doi: 10.1186/1756-9966-29-13
Figure Lengend Snippet: Detection of IGFBP7, caspase-3, VEGF, and apoptosis expressed in homeograft tumors sections with original magnification × 100 in A-F, and ×400 in G-L . A shows significantly higher IGFBP7 expression in pcDNA3.1-IGFBP7. B demonstrates the successful transfection of pcDNA3.1 plasmid. C shows the physiological expression of IGFBP7 in melanoma (red color, as blue arrows indicate). D-F shows the effect of pcDNA3.1-IGFBP7 on caspase-3 expression in the cytoplasm of tumor sections, with strong expression in pcDNA3.1-IGFBP7 group seen in D, while weak expression in the pcDNA3.1-CONTROL and B16-F10 cell groups seen in E, F. G-I shows the expression of VEGF in vivo , with negative expression in most of cells in the pcDNA3.1-IGFBP7 group seen in G, while strong expression in the cytoplasm of pcDNA3.1-CONTROL and B16-F10 cell groups (red arrow represented) showed in H, I. J-L shows tumor apoptosis in vivo , with few apoptotic cells in pcDNA3.1-CONTROL (K) and B16-F10 cell groups (L), while increased apoptosis in the B16-F10 tissue in mice treated with pcDNA3.1-IGFBP7 (J) (red arrow shows deep blue cells).
Article Snippet:
Techniques: Expressing, Transfection, Plasmid Preparation, Control, In Vivo